pLV-Puro-{CMV-CuO}>{PAX3::FOXO1}:T2A:mCherry
(Plasmid #236629)

• Purpose: Puromycin selected lentiviral construct with cumate-inducible PAX3::FOXO1
• Depositing Lab: Genevieve Kendall
• Publication: Kent et al Sci Rep. 2024 Sep 5;14(1):20773. doi: 10.1038/s41598-024-71227-3.

Backbone

• Vector backbone: Mammalian Tet Inducible Gene Expression Lentiviral Vector
• Backbone manufacturer: VectorBuilder
• Backbone size w/o insert (bp): 6700
• Total vector size (bp): 11134
• Modifications to backbone: Replaced the TRE sequence with a CMV promoter and a CuO sequence; added the coding sequence for human PAX3::FOXO1 with no stop codon directly downstream of TRE linked to mCherry with a T2A sequence; added a puromycin resistance gene for antibiotic selection
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: PAX3::FOXO1
• Species: H. sapiens (human)
• Insert Size (bp): 2508
• Entrez Gene: FOXO1 (a.k.a. FKH1, FKHR, FOXO1A)
• Entrez Gene: PAX3 (a.k.a. CDHS, HUP2, PAX-3, WS1, WS3)
• Promoter: CMV-CuO

Cloning Information for Gene/Insert 1

• Cloning method: Gene Synthesis
• 5′ sequencing primer: N/A

Gene/Insert 2

• Gene/Insert name: mCherry
• Species: Synthetic
• Insert Size (bp): 711
• Promoter: CMV-CuO

Cloning Information for Gene/Insert 2

• Cloning method: Gene Synthesis
• 5′ sequencing primer: N/A

Resource Information

• Supplemental Documents:
  • ptz1056 pLV-Puro-CMV-CuO_P3F-T2A-mCherry.gb
• A portion of this plasmid was derived from a plasmid made by: VectorBuilder

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry