pLV-Hygro-TRE>hHES3:T2A:EGFP
(Plasmid #236630)

• Purpose: Hygromycin selected lentiviral construct with tetracyclin-inducible human HES3
• Depositing Lab: Genevieve Kendall
• Publication: Kent et al Sci Rep. 2024 Sep 5;14(1):20773. doi: 10.1038/s41598-024-71227-3.

Backbone

• Vector backbone: Mammalian Tet Inducible Gene Expression Lentiviral Vector
• Backbone manufacturer: VectorBuilder
• Backbone size w/o insert (bp): 6700
• Total vector size (bp): 9507
• Modifications to backbone: Added the coding sequence for human HES3 with no stop codon directly downstream of the TRE sequence, linked to EGFP with a T2A sequence; added a hygromycin resistance gene for antibiotic selection
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: HES3
• Species: H. sapiens (human)
• Insert Size (bp): 558
• GenBank ID: NM_001024598.3
• Entrez Gene: HES3 (a.k.a. bHLHb43)
• Promoter: TRE

Cloning Information for Gene/Insert 1

• Cloning method: Gene Synthesis
• 5′ sequencing primer: N/A

Gene/Insert 2

• Gene/Insert name: EGFP
• Species: Aequorea victoria
• Insert Size (bp): 720
• Mutation: Codon optimized based on a variant of wildtype GFP from the jellyfish Aequorea victoria
• Promoter: TRE

Cloning Information for Gene/Insert 2

• Cloning method: Gene Synthesis
• 5′ sequencing primer: N/A

Resource Information

• Supplemental Documents:
  • ptz1057 pLV-Hygro-TRE_hHES3-T2A-EGFP.gb
• A portion of this plasmid was derived from a plasmid made by: VectorBuilder

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry