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Addgene

pVID
(Plasmid #236635)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 236635 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pVID
  • Backbone manufacturer
    Evelyne Ruchti and Brian McCabe
  • Modifications to backbone
    added dU6-3 promoter for gRNA, Multiple cloning site
  • Vector type
    CRISPR
  • Promoter dU6:3
  • Selectable markers
    3xP3-ZsGreen

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid contains Drosophila U6:3 promoter sequence for gRNA expression followed by a multiple cloning site. The backbone includes a ZsGreen fluorescent protein under 3xP3 promoter to allow detection of undesired vector backbone insertion that can occur during HDR.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pVID was a gift from Brian McCabe (Addgene plasmid # 236635 ; http://n2t.net/addgene:236635 ; RRID:Addgene_236635)
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