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pCMV5D HA-PPM1H_M flap
(Plasmid #236718)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 236718 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV5D
  • Backbone size w/o insert (bp) 4987
  • Total vector size (bp) 6565
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PPM1H_M flap
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1578
  • Mutation
    PPM1H flap domain replaced with PPM1M flap domain
  • Entrez Gene
    PPM1H (a.k.a. ARHCL1, NERPP-2C, URCC2)
  • Entrez Gene
    PPM1M (a.k.a. PP2C-eta, PP2CE, PP2Ceta)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV5D HA-PPM1H_M flap was a gift from Suzanne Pfeffer (Addgene plasmid # 236718 ; http://n2t.net/addgene:236718 ; RRID:Addgene_236718)

  • For your References section:

    PPM1M, a LRRK2-counteracting, phosphoRab12-preferring phosphatase with potential link to Parkinson’s disease. Claire Y. Chiang, Neringa Pratuseviciute, Yu-En Lin, Ayan Adhikari, Wondwossen M. Yeshaw, Chloe Flitton, Pemba L. Sherpa, Francesca Tonelli, Irena Rektorova, Timothy Lynch, Joanna Siuda, Monika Rudzińska-Bar, Oleksandr Pulyk, Peter Bauer, Christian Beetz, Dennis W. Dickson10, Owen A. Ross, Zbigniew K. Wszolek, Christine Klein, Alexander Zimprich, Dario R. Alessi, Esther M. Sammler and Suzanne R. Pfeffer. bioRxiv, 2025 10.1101/2025.03.19.644182
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