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Addgene

PB-TA-ERN-WDR5-FLAG
(Plasmid #236721)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 236721 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PB-TA-ERN
  • Modifications to backbone
    FLAG tag addition
  • Vector type
    Mammalian Expression ; piggyBac transposon
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    WDR5
  • Species
    H. sapiens (human)
  • Entrez Gene
    WDR5 (a.k.a. BIG-3, BIG3, CFAP89, SWD3)
  • Tag / Fusion Protein
    • FLAG (C terminal on backbone)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PB-TA-ERN-WDR5-FLAG was a gift from Tadashi Nakagawa (Addgene plasmid # 236721 ; http://n2t.net/addgene:236721 ; RRID:Addgene_236721)

  • For your References section:

    KBG syndrome-associated protein ANKRD11 regulates SETD5 expression to modulate rRNA levels and translation. Sashiyama S, Nakagawa T, Nakagawa M, Hosogane M, Watanabe Y, Ashitomi H, Yamane K, Shibuya N, Moroishi T, Nakayama K, Hosoi T. iScience 2025 May 19, 112699 10.1016/j.isci.2025.112699
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