iC30 PB-Zim3-dCas9-P2A-BFP_hygro
(Plasmid
#236743)
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PurposeConstitutive expression of Zim3-dCas9
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 236743 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonePB-CAG-WPRE_EF1a-WPRE
- Total vector size (bp) 14972
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameCAG-Zim3-NLS-dCas9-HA-P2A-2xmycNLS-tagBFP-WPRE
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SpeciesSynthetic
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Insert Size (bp)7829
- Promoter CAG
Cloning Information for Gene/Insert 1
- Cloning method Gibson Cloning
- 5′ sequencing primer gttcggcttctggcgtgt (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameEF1a-hygro-WPRE
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Insert Size (bp)2803
- Promoter EF1a
Cloning Information for Gene/Insert 2
- Cloning method Gibson Cloning
- 5′ sequencing primer ttccatttcaggtgtcgtgA (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
iC30 PB-Zim3-dCas9-P2A-BFP_hygro was a gift from iPSC Neurodegenerative Disease Initiative (iNDI) (Addgene plasmid # 236743 ; http://n2t.net/addgene:236743 ; RRID:Addgene_236743)