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Addgene

pBTK1129
(Plasmid #236752)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 236752 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pBTK1001
  • Backbone size w/o insert (bp) 1747
  • Total vector size (bp) 2703
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Type 2-3 GFP cassette
  • Species
    Synthetic
  • Insert Size (bp)
    956
  • Promoter CP25

Cloning Information

  • Cloning method Golden Gate
  • 5′ sequencing primer GCATCGTCTCATCGGTCTCAAACGctttggcagtttattcttgac
  • 3′ sequencing primer ATGCCGTCTCAGGTCTCAGGATtgacaaatgctctttcccta
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBTK1129 was a gift from Jeffrey Barrick (Addgene plasmid # 236752 ; http://n2t.net/addgene:236752 ; RRID:Addgene_236752)

  • For your References section:

    One-step genome engineering in bee gut bacterial symbionts. Lariviere PJ, Ashraf AHMZ, Navarro-Escalante L, Leonard SP, Miller LG, Moran NA, Barrick JE. mBio. 2024 Sep 11;15(9):e0139224. doi: 10.1128/mbio.01392-24. Epub 2024 Aug 6. 10.1128/mbio.01392-24 PubMed 39105596
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