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LgBiT-dSaCas9
(Plasmid #236758)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 236758 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pCDNA
  • Total vector size (bp) 9403
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NanoLuc LgBit
  • Alt name
    NanoLuc luciferase
  • Insert Size (bp)
    474
  • Promoter CMV
  • Tag / Fusion Protein
    • dSaCas9

Cloning Information

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LgBiT-dSaCas9 was a gift from David Segal (Addgene plasmid # 236758 ; http://n2t.net/addgene:236758 ; RRID:Addgene_236758)

  • For your References section:

    A split luciferase biosensing platform for detection and imaging of chromatin loops in individual live cells. Heath NG, Gomez JA, McGinty SP, O'Geen H, Segal DJ. Nucleic Acids Res. 2025 Nov 26;53(22):gkaf1324. doi: 10.1093/nar/gkaf1324. 10.1093/nar/gkaf1324 PubMed 41335466
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