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PB-TRE3G-dN40-TP53-CCRB
(Plasmid #236774)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 236774 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PB-TRE3G-CCRB
  • Backbone manufacturer
    Synthetic
  • Backbone size w/o insert (bp) 10420
  • Total vector size (bp) 11485
  • Vector type
    Mammalian Expression ; PiggyBac
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TP53
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1182
  • Mutation
    deleted amino acids 1-40
  • GenBank ID
    NM_000546.6
  • Entrez Gene
    TP53 (a.k.a. BCC7, BMFS5, LFS1, P53, TRP53)
  • Promoter TRE3G promoter

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The C to G SNP (rs1042522) in the TP53 insert makes the coding amino acid change from proline to arginine (p.P33R). This SNP is common in all world populations.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PB-TRE3G-dN40-TP53-CCRB was a gift from Kazutoshi Takahashi (Addgene plasmid # 236774 ; http://n2t.net/addgene:236774 ; RRID:Addgene_236774)

  • For your References section:

    EIF3D safeguards the homeostasis of key signaling pathways in human primed pluripotency. Okubo C, Nakamura M, Sato M, Shichino Y, Mito M, Takashima Y, Iwasaki S, Takahashi K. Sci Adv. 2025 Apr 11;11(15):eadq5484. doi: 10.1126/sciadv.adq5484. Epub 2025 Apr 9. 10.1126/sciadv.adq5484 PubMed 40203091
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