pTB105
(Plasmid
#236778)
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Purpose(Empty Backbone) Backbone for cloning sgRNAs to be used with CRISPR Cas9 cytosine base editor. Expressed from the 18S rRNA SSU locus in Leishmania. Contains puromycin selection marker.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 236778 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepUC19
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Vector typeCRISPR
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTB105 was a gift from Tom Beneke (Addgene plasmid # 236778 ; http://n2t.net/addgene:236778 ; RRID:Addgene_236778) -
For your References section:
Improved base editing and functional screening in Leishmania via co-expression of the AsCas12a ultra variant, a T7 RNA polymerase, and a cytosine base editor. Herrmann May N, Cao A, Schmid A, Link F, Arias-Del-Angel J, Meiser E, Beneke T. eLife. 2025 Feb 24;13:RP97437. doi: 10.7554/eLife.97437. 10.7554/eLife.97437 PubMed 39991929