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pTB107-Phleo
(Plasmid #236782)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 236782 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pUC19
  • Backbone size w/o insert (bp) 2460
  • Vector type
    CRISPR
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    EPI300
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    hyBE4max (CBE); Cas12a (Acidaminococcus sp. BV3L6)-P2A-T7 RNAP
  • Species
    APOBEC-1 (rat), RAD51 ssDNA DBD (L. major), Cas9 (S. pyogenes), UGI (B. subtilis bacteriophage)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTB107-Phleo was a gift from Tom Beneke (Addgene plasmid # 236782 ; http://n2t.net/addgene:236782 ; RRID:Addgene_236782)

  • For your References section:

    Improved base editing and functional screening in Leishmania via co-expression of the AsCas12a ultra variant, a T7 RNA polymerase, and a cytosine base editor. Herrmann May N, Cao A, Schmid A, Link F, Arias-Del-Angel J, Meiser E, Beneke T. eLife. 2025 Feb 24;13:RP97437. doi: 10.7554/eLife.97437. 10.7554/eLife.97437 PubMed 39991929
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