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MCUb-MCU-NTD-pLYS1-Flag
(Plasmid #236784)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 236784 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLYS1
  • Backbone size w/o insert (bp) 7328
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    Room Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MCU-MCUB chimera
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1134
  • Mutation
    Replaced MCUB N-terminal domain (aa 1-170) with MCU N-terminal domain (aa 1-185)
  • Entrez Gene
    MCU (a.k.a. C10orf42, CCDC109A, HsMCU)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.11.25.625228 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MCUb-MCU-NTD-pLYS1-Flag was a gift from Dipayan Chaudhuri (Addgene plasmid # 236784 ; http://n2t.net/addgene:236784 ; RRID:Addgene_236784)

  • For your References section:

    Mechanism of MCUB-Dependent Inhibition of Mitochondrial Calcium Uptake. Rai NK, Eberhardt DR, Balynas AM, MacEwen MJS, Bratt AR, Sancak Y, Chaudhuri D. J Cell Physiol. 2025 Apr;240(4):e70033. doi: 10.1002/jcp.70033. 10.1002/jcp.70033 PubMed 40227803
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