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pNL [NlucP/CRE/Hygro] Vector
(Plasmid #236829)

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Item Catalog # Description Quantity Price (USD)
Plasmid 236829 Standard format: Plasmid sent in bacteria as agar stab 1 $94 *
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DNA 236829-D20 20 μg of DNA in Tris buffer $413 *
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DNA 236829-D50 50 μg of DNA in Tris buffer $495 *
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DNA 236829-D100 100 μg of DNA in Tris buffer $585 *
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Backbone

  • Vector backbone
    pNL1.1.CMV[Nluc/CMV] Vector
  • Vector type
    Luciferase
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CRE
  • Species
    H. sapiens (human)
  • Mutation
    None
  • Promoter minP
  • Tag / Fusion Protein
    • NanoLuc (R) (N terminal on backbone)

Cloning Information

  • Cloning method Other

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that this plasmid exists as a multimer. This is not expected to impact the end function of the plasmid. Please refer to the Addgene Blog post on multimers for more information: https://blog.addgene.org/plasmids-101-dimers-and-multimers

Purpose

Ready-to-use DNA prep. DNA aliquots are suitable for use in molecular biology and scientific research.

Delivery

  • Amount 20 μg
  • Concentration 1 μg/μL
  • Pricing $413 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Addgene does not test for endotoxin levels on these samples.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNL [NlucP/CRE/Hygro] Vector was a gift from Promega Corporation (Addgene plasmid # 236829 ; http://n2t.net/addgene:236829 ; RRID:Addgene_236829)
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