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NanoBRET BiBRET Vector, NanoLuc-HRAS WT-CRAF RBD-HaloTag
(Plasmid #236861)

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Item Catalog # Description Quantity Price (USD)
Plasmid 236861 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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DNA 236861-D20 20 μg of DNA in Tris buffer $393 *
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Backbone

  • Vector backbone
    pFC221K HaloTag(R) CMV-Blast BiBRET Vector and pFN224C NanoLuc(R) CMV-Neo BiBRET-Balanced Vector
  • Vector type
    Mammalian Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    HRAS
  • Species
    H. sapiens (human)
  • Mutation
    None
  • Entrez Gene
    HRAS (a.k.a. C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras)
  • Promoter CMV
  • Tag / Fusion Protein
    • NanoLuc (R) (N terminal on backbone)

Cloning Information for Gene/Insert 1

  • Cloning method Other

Gene/Insert 2

  • Gene/Insert name
    cRAF(RBD)
  • Species
    H. sapiens (human)
  • Entrez Gene
    RAF1 (a.k.a. CMD1NN, CRAF, NS5, Raf-1, c-Raf)
  • Tag / Fusion Protein
    • HaloTag (R) (C terminal on backbone)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Information for DNA (Catalog # 236861-D20) ( Back to top)

Purpose

DNA aliquots are suitable for use in molecular biology and scientific research

Delivery

  • Amount 20 μg
  • Guaranteed Concentration 500 ng/μl +/- 25 ng/μl
  • Pricing $393 USD
  • Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).

Terms and Licenses

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Review the sequencing results. Addgene does not test for endotoxin levels on these samples.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NanoBRET BiBRET Vector, NanoLuc-HRAS WT-CRAF RBD-HaloTag was a gift from Promega Early Access Program (Addgene plasmid # 236861 ; http://n2t.net/addgene:236861 ; RRID:Addgene_236861)
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