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Addgene

NanoLuc-NRAS-CRAF(RBD)-HaloTag BiBRET Vector
(Plasmid #236864)

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Item Catalog # Description Quantity Price (USD)
Plasmid 236864 Standard format: Plasmid sent in bacteria as agar stab 1 $89 *

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Backbone

  • Vector backbone
    pFC221K HaloTag(R) CMV-Blast BiBRET Vector and pFN224C NanoLuc(R) CMV-Neo BiBRET-Balanced Vector
  • Vector type
    Mammalian Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    NRAS
  • Species
    H. sapiens (human)
  • Mutation
    Ras Binding Domain
  • Entrez Gene
    NRAS (a.k.a. ALPS4, CMNS, KRAS, N-ras, NCMS, NRAS1, NS6)
  • Promoter CMV
  • Tags / Fusion Proteins
    • NanoLuc (R) (N terminal on backbone)
    • HaloTag (R) (C terminal on backbone)

Cloning Information for Gene/Insert 1

  • Cloning method Other

Gene/Insert 2

  • Gene/Insert name
    cRAF(RBD)
  • Species
    H. sapiens (human)
  • Entrez Gene
    RAF1 (a.k.a. CMD1NN, CRAF, NS5, Raf-1, c-Raf)
  • Tag / Fusion Protein
    • HaloTag (R) (C terminal on backbone)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NanoLuc-NRAS-CRAF(RBD)-HaloTag BiBRET Vector was a gift from Promega Corporation (Addgene plasmid # 236864)
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