BiBiT/SmBiT-PRKACA/LgBiT-PRKAR2A/BlastR Vector
(Plasmid
#237156)
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PurposeExpress SmBiT-PRKACA/LgBiT-PRKAR2A Fusion Protein in Mammalian Cells under a CMV promoter
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 237156 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 * |
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Backbone
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Vector backbonepFN217K LgBiT CMV-Hyg Flexi Vector and pFN218K SmBiT CMV-Blast Flexi Vector
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Vector typeMammalian Expression
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert namePRKACA
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SpeciesH. sapiens (human)
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MutationNone
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Entrez GenePRKACA (a.k.a. CAFD1, PKACA, PPNAD4)
- Promoter CMV
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Tag
/ Fusion Protein
- SmBiT (N terminal on backbone)
Cloning Information for Gene/Insert 1
- Cloning method Other
Gene/Insert 2
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Gene/Insert namePRKAR2A
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SpeciesH. sapiens (human)
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Entrez GenePRKAR2A (a.k.a. PKR2, PRKAR2)
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Tag
/ Fusion Protein
- LgBiT (N terminal on backbone)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
BiBiT/SmBiT-PRKACA/LgBiT-PRKAR2A/BlastR Vector was a gift from Promega Corporation (Addgene plasmid # 237156)