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Addgene

EGFR(L858R,T790M)-NanoLuc Fusion Vector
(Plasmid #237199)

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Item Catalog # Description Quantity Price (USD)
Plasmid 237199 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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DNA 237199-D20 20 μg of DNA in Tris buffer $393 *
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Backbone

  • Vector backbone
    pFC32K Nluc CMV-neo Flexi(R) Vector
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EGFR
  • Species
    H. sapiens (human)
  • Mutation
    L858R/T790M double mutations
  • Entrez Gene
    EGFR (a.k.a. ERBB, ERBB1, ERRP, HER1, NISBD2, NNCIS, PIG61, mENA)
  • Promoter CMV
  • Tag / Fusion Protein
    • NanoLuc (R) (C terminal on backbone)

Cloning Information

  • Cloning method Other

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Information for DNA (Catalog # 237199-D20) ( Back to top)

Purpose

DNA aliquots are suitable for use in molecular biology and scientific research

Delivery

  • Amount 20 μg
  • Guaranteed Concentration 500 ng/μl +/- 25 ng/μl
  • Pricing $393 USD
  • Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).

Terms and Licenses

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Review the sequencing results. Addgene does not test for endotoxin levels on these samples.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EGFR(L858R,T790M)-NanoLuc Fusion Vector was a gift from Promega Early Access Program (Addgene plasmid # 237199 ; http://n2t.net/addgene:237199 ; RRID:Addgene_237199)
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