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Addgene

pET-hIRF5-V5(4D)-LPETG
(Plasmid #237225)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 237225 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pET
  • Total vector size (bp) 6784
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Depositing lab uses TOP10F' strain for protein expression.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Interferon regulatory factor 5
  • Species
    H. sapiens (human)
  • Mutation
    S451D, S453D, S456D, S462D
  • Entrez Gene
    IRF5 (a.k.a. SLEB10)
  • Tag / Fusion Protein
    • 6xHis (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-hIRF5-V5(4D)-LPETG was a gift from Elliot Chaikof (Addgene plasmid # 237225 ; http://n2t.net/addgene:237225 ; RRID:Addgene_237225)

  • For your References section:

    Self-assembling protein nanoparticles for cytosolic delivery of nucleic acids and proteins. Eweje F, Ibrahim V, Shajii A, Walsh ML, Ahmad K, Alrefai A, Miyasato D, Davis JR, Ham H, Li K, Roehrl M, Haller CA, Liu DR, Chen J, Chaikof EL. Nat Biotechnol. 2025 May 15. doi: 10.1038/s41587-025-02664-2. 10.1038/s41587-025-02664-2 PubMed 40374955
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