pTW335i3
(Plasmid
#237323)
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Purposeexpresses N. tabacum RbcL RbcS-6xHis
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 237323 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBR322
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)S2060
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameN. tabacum RbcL RbcS-6xHis
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SpeciesN. tabacum
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Tag
/ Fusion Protein
- 6xHis (C terminal on insert)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byLin MT, Stone WD, Chaudhari V, Hanson MR. 2020. Small subunits can determine enzyme kinetics of tobacco Rubisco expressed in Escherichia coli. Nature Plants 6, 1289–1299.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTW335i3 was a gift from Tina Wang (Addgene plasmid # 237323 ; http://n2t.net/addgene:237323 ; RRID:Addgene_237323)