pUC18 3xFLAG COP1
(Plasmid
#237334)
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Purposeexpression in bacterial cells for protein purification
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 237334 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC18
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCOP1
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SpeciesH. sapiens (human)
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Entrez GeneCOP1 (a.k.a. CFAP78, FAP78, RFWD2, RNF200)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUC18 3xFLAG COP1 was a gift from Michele Pagano (Addgene plasmid # 237334 ; http://n2t.net/addgene:237334 ; RRID:Addgene_237334) -
For your References section:
Cryptochromes-Mediated Inhibition of the CRL4(Cop1)-Complex Assembly Defines an Evolutionary Conserved Signaling Mechanism. Rizzini L, Levine DC, Perelis M, Bass J, Peek CB, Pagano M. Curr Biol. 2019 Jun 17;29(12):1954-1962.e4. doi: 10.1016/j.cub.2019.04.073. Epub 2019 May 30. 10.1016/j.cub.2019.04.073 PubMed 31155351