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pTCB-avelHCknob6xHis
(Plasmid #237383)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 237383 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pTwist CMV BetaGlobin
  • Backbone manufacturer
    Twist Bioscience
  • Backbone size w/o insert (bp) 4832
  • Total vector size (bp) 6260
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    avelumab heavy chain
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1428
  • Promoter CMV
  • Tag / Fusion Protein
    • 6xHis (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site NheI (not destroyed)
  • 5′ sequencing primer N/A
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2022.10.26.513931 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTCB-avelHCknob6xHis was a gift from Carolyn Bertozzi & Jessica Stark (Addgene plasmid # 237383 ; http://n2t.net/addgene:237383 ; RRID:Addgene_237383)

  • For your References section:

    Antibody-lectin chimeras for glyco-immune checkpoint blockade. Stark JC, Gray MA, Ibarlucea-Benitez I, Lustig M, Bond A, Cho B, Govil I, Luu T, Priestley MJ, Veth TS, Errington WJ, Bruncsics B, Ribi MK, Williams LA, Sarkar CA, Wisnovsky S, Riley NM, Morrissey MA, Valerius T, Ravetch JV, Bertozzi CR.. Nat Biotechnol 2025 10.1038/s41587-025-02884-6
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