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Addgene

MIGR1-U6gRNA1-Filler-v1
(Plasmid #237399)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 237399 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MIGR1
  • Backbone manufacturer
    Warren Pear (Addgene #27490)
  • Backbone size (bp) 5100
  • Modifications to backbone
    Damage a BspMI site
  • Vector type
    Mammalian Expression, Retroviral, CRISPR
  • Promoter U6, PGK

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This vector is designed for dual sgRNA cloning to delete cis-regulatory elements when paired with MIGR1-U6gRNA1-Filler-v2 (237400). It enables direct transfer of the U6gRNA cassette (containing the inserted gRNA) between vectors by restriction digestion and ligation. It can also be used for individual sgRNA cloning.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MIGR1-U6gRNA1-Filler-v1 was a gift from Zuoming Sun (Addgene plasmid # 237399 ; http://n2t.net/addgene:237399 ; RRID:Addgene_237399)

  • For your References section:

    Protocol for CRISPR-mediated deletion of cis-regulatory element in murine Th17 cells for in vivo assessment of effector function. Zhong X, Wu H, Wang G, Sun Z. STAR Protoc. 2025 Jun 20;6(2):103831. doi: 10.1016/j.xpro.2025.103831. Epub 2025 May 20. 10.1016/j.xpro.2025.103831 PubMed 40397576
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