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FLAG-sc-WDR59-SEC13
(Plasmid #237416)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 237416 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pRK5
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    WDR59-SEC13 fusion
  • Species
    H. sapiens (human)
  • Entrez Gene
    WDR59 (a.k.a. CDW12, FP977, p90-120)
  • Entrez Gene
    SEC13 (a.k.a. D3S1231E, SEC13L1, SEC13R, npp-20)
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Contains two fused inserts: human SEC13 fused within human WDR59

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FLAG-sc-WDR59-SEC13 was a gift from David Sabatini (Addgene plasmid # 237416 ; http://n2t.net/addgene:237416 ; RRID:Addgene_237416)

  • For your References section:

    Structural basis for the dynamic regulation of mTORC1 by amino acids. Valenstein ML, Wranik M, Lalgudi PV, Linde-Garelli KY, Choi Y, Chivukula RR, Sabatini DM, Rogala KB. Nature. 2025 Aug 20. doi: 10.1038/s41586-025-09428-7. 10.1038/s41586-025-09428-7 PubMed 40836086
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