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Addgene

CASTOR1-HA (D304A)
(Plasmid #237419)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 237419 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pRK5
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CASTOR1
  • Species
    H. sapiens (human)
  • Mutation
    D304A
  • Entrez Gene
    GATSL3 (a.k.a. CASTOR1)
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Based on Addgene Plasmid # 84487 (pRK5 CASTOR1-HA Cterm) from Chantranupong et al. 2016

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CASTOR1-HA (D304A) was a gift from David Sabatini (Addgene plasmid # 237419 ; http://n2t.net/addgene:237419 ; RRID:Addgene_237419)

  • For your References section:

    Structural basis for the dynamic regulation of mTORC1 by amino acids. Valenstein ML, Wranik M, Lalgudi PV, Linde-Garelli KY, Choi Y, Chivukula RR, Sabatini DM, Rogala KB. Nature. 2025 Aug 20. doi: 10.1038/s41586-025-09428-7. 10.1038/s41586-025-09428-7 PubMed 40836086
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