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pGEMHE-MmCENPT-4NS-GFP
(Plasmid #237431)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 237431 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEMHE
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mus musculus CENP-T with 4 rat-specific residues in HFD
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1545
  • Mutation
    432 T to A; 463 I to T, 470 L to E, 485 Q to L
  • Entrez Gene
    Cenpt (a.k.a. G630055P03Rik)
  • Promoter T7
  • Tag / Fusion Protein
    • GFP (C terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: This plasmid contains a D36N mutation in GFP. This mutation is not known to impact GFP fluorescence. Please see https://pubmed.ncbi.nlm.nih.gov/27193686/ for details.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEMHE-MmCENPT-4NS-GFP was a gift from Michael Lampson (Addgene plasmid # 237431 ; http://n2t.net/addgene:237431 ; RRID:Addgene_237431)

  • For your References section:

    Adaptive evolution of CENP-T modulates centromere binding. Dudka D, Nguyen AL, Boese KG, Marescal O, Akins RB, Black BE, Cheeseman IM, Lampson MA. Curr Biol. 2025 Mar 10;35(5):1012-1022.e5. doi: 10.1016/j.cub.2025.01.017. Epub 2025 Feb 12. 10.1016/j.cub.2025.01.017 PubMed 39947176
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