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pLCHKOv4
(Plasmid #237451)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 237451 Standard format: Plasmid sent in bacteria as agar stab 1 $89

Backbone

  • Vector backbone
    pLCHKOv3
  • Backbone manufacturer
    Thomas Gonatopoulos-Pournatzis (Addgene plasmid # 209025)
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    ccdB Survival
  • Growth instructions
    Carrying ccdB gene as stuffer that is replaced during guide cloning. After cloning guides, use NEB Stable bacterial growth strains to amplify plasmid.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Guide expression cassettes contain 10x Genomics capture sequence 2 (CS2), tevopreQ1 riboswitch, and BveI cloning sites.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLCHKOv4 was a gift from Thomas Gonatopoulos-Pournatzis (Addgene plasmid # 237451)
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