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pLCHKOv5
(Plasmid #237452)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 237452 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pLCHKOv3
  • Backbone manufacturer
    Thomas Gonatopoulos-Pournatzis (Addgene plasmid # 209025)
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

These hgRNAs are processed by Cas13b to cleave the capped 5' end and by Cas12a to remove the polyadenylated 3' end (nucleases not encoded on this plasmid). The vector contains BveI cloning sites and includes a puromycin resistance marker for selection.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLCHKOv5 was a gift from Thomas Gonatopoulos-Pournatzis (Addgene plasmid # 237452 ; http://n2t.net/addgene:237452 ; RRID:Addgene_237452)
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