pcDNA5-3xFLAG-NRF1-∆ex7_WobbleMut
(Plasmid #237456)

• Purpose: Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of 3xFlag-tagged NRF1 isoform lacking the alternative exon-7 (∆ex7) mutated to be resistant to NRF1 siRNA
• Depositing Lab: Thomas Gonatopoulos-Pournatzis
• Publication: Single-Cell Exon Deletion Profiling Reveals Splicing Events That Shape Gene Expression and Cell State Dynamics (unpublished)

Backbone

• Vector backbone: pcDNA5/FRT/TO
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin, only when integrated into FlpIn FRT site

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NRF1 ∆exon-7
• Species: H. sapiens (human)
• Promoter: CMV/TO inducible promoter
• Tag / Fusion Protein:
  • 3xFLAG (N terminal on insert)

Cloning Information

• Cloning method: Golden Gate

Resource Information

• Supplemental Documents:
  • pcDNA5-3xFLAG-NRF1-∆ex7_WobbleMut.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry