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Addgene

pBAD42-BtuCEDFB
(Plasmid #237541)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 237541 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pBAD42
  • Backbone manufacturer
    Yale Coli Genetic Stock Center
  • Backbone size w/o insert (bp) 6236
  • Total vector size (bp) 11150
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Depositing lab also uses BL21 (DE3) for protein expression.
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    btuB
  • Alt name
    btuB
  • Species
    E. coli
  • Insert Size (bp)
    1842
  • Promoter pBAD

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer 5′-CGC GGC GTC GGT ACC ATG ATT AAA AAA GCT TCG CTG CTG ACG GCG-3'
  • 3′ sequencing primer 5′-CGC GGC GTC TCT AGA TTA GAA GGT GTA GCT GCC AGA CAA GGT G-3′
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    btuF
  • Species
    E. coli
  • Insert Size (bp)
    798
  • Promoter pBAD

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer 5′-CGC GGC GTC CTC GAG ATG GCT AAG TCA CTG TTC AGG GCG CTG GTC-3′
  • 3′ sequencing primer 5′-CGC GGC GTC TCT AGA CGG TAG GAC GGT ACC TCA ATC TAC CTG TGA AAG CGC ATT ACA GAG-3'
    • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
    btuCED
  • Alt name
    btuCED operon
  • Species
    E. coli
  • Insert Size (bp)
    2274
  • Promoter pBAD

Cloning Information for Gene/Insert 3

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer 5′-CGC GGC GTC GAA TTC ATG CTG ACA CTT GCC CGC CAA CAA CAG CG-3′
  • 3′ sequencing primer 5′-CGC GGC GTC TCT AGA CGG TAG GAC CTC GAG TCA GAT GGT CGA AAT CAG CAT TCT GTG ACC-3′
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBAD42-BtuCEDFB was a gift from Squire Booker (Addgene plasmid # 237541 ; http://n2t.net/addgene:237541 ; RRID:Addgene_237541)

  • For your References section:

    Enhanced Solubilization of Class B Radical S-Adenosylmethionine Methylases by Improved Cobalamin Uptake in Escherichia coli. Lanz ND, Blaszczyk AJ, McCarthy EL, Wang B, Wang RX, Jones BS, Booker SJ. Biochemistry. 2018 Mar 6;57(9):1475-1490. doi: 10.1021/acs.biochem.7b01205. Epub 2018 Feb 19. 10.1021/acs.biochem.7b01205 PubMed 29298049
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