TDP-43 F147/149L GFP
(Plasmid
#237545)
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PurposeExpression of GFP-tag fused to mutant TDP-43 F147/19L
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 237545 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-C1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGFP-tagged TDP-43 F147/149L
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SpeciesH. sapiens (human)
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MutationTDP-43 F147L and F149L
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Entrez GeneTARDBP (a.k.a. ALS10, TDP-43)
- Promoter CMV
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Tag
/ Fusion Protein
- EGFP (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
TDP-43 F147/149L GFP was a gift from Tatyana Shelkovnikova (Addgene plasmid # 237545 ; http://n2t.net/addgene:237545 ; RRID:Addgene_237545)