Topo_SpCas9.tracrRNA-CS1_AsCas12a DRv10
(Plasmid
#237553)
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PurposeTOPO vector for the cloning of the SpCas9 tracrRNA-CS1 - AsCas12a Direct Repeat (DR) v10 fragment into the pLCHKO_v4 hgRNA vector for scCHyMErA-Seq experiments.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 237553 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backboneTOPO
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSpCas9.tracrRNA-CS1_AsCas12a DRv10
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SpeciesAcidaminococcus sp. BV3L6, Streptococcus pyogenes
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer GTAAAACGACGGCCAGT
- 3′ sequencing primer CAGGAAACAGCTATGAC
- (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Guide expression cassettes contain 10x Genomics capture sequence 2 (CS2), tevopreQ1 riboswitch, and BveI cloning sites.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Topo_SpCas9.tracrRNA-CS1_AsCas12a DRv10 was a gift from Thomas Gonatopoulos-Pournatzis (Addgene plasmid # 237553 ; http://n2t.net/addgene:237553 ; RRID:Addgene_237553)
Map uploaded by the depositor.
