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pAG8Ha-His-PPIL1
(Plasmid #237554)

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Item Catalog # Description Quantity Price (USD)
Plasmid 237554 Standard format: Plasmid sent in bacteria as agar stab 1 $89 *
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Backbone

  • Vector backbone
    pET11
  • Backbone size w/o insert (bp) 4500
  • Total vector size (bp) 4824
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Peptidyl-prolyl cis-trans isomerase-like 1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    498
  • Entrez Gene
    PPIL1 (a.k.a. CGI-124, CYPL1, PCH14, PPIase, hCyPX)
  • Promoter T7
  • Tag / Fusion Protein
    • 8xHis - TEV cleavage site (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KasI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 term
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

insert was synthesized and is codon optimized for E. coli expression

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAG8Ha-His-PPIL1 was a gift from David Libich (Addgene plasmid # 237554 ; http://n2t.net/addgene:237554 ; RRID:Addgene_237554)

  • For your References section:

    The Spliceosomal Peptidyl Prolyl Isomerase Like 1 Interacts with the Low-Complexity Domain of the RNA Binding Protein EWS Modulating Its Phase Separation Behavior. Parra GL, Sohn EJ, Xu X, Libich DS. Biochemistry. 2025 Aug 5;64(15):3173-3177. doi: 10.1021/acs.biochem.5c00193. Epub 2025 Jul 16. 10.1021/acs.biochem.5c00193 PubMed 40668764
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