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PB-2G-CNCB_sgRBBP6-68
(Plasmid #237555)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 237555 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PB-2G-CNCB
  • Backbone manufacturer
    Synthetic
  • Backbone size w/o insert (bp) 8714
  • Total vector size (bp) 8730
  • Modifications to backbone
    The RBBP6 sgRNAs #6 and #8 were inserted into the Esp3I and BbsI sites of PB-2G-CNCB, respectively.
  • Vector type
    Mammalian Expression ; PiggyBac
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RBBP6
  • gRNA/shRNA sequence
    TCGGACGAGCGCCCCACTG
  • Species
    H. sapiens (human)
  • GenBank ID
    NM_006910.5
  • Entrez Gene
    RBBP6 (a.k.a. MY038, P2P-R, PACT, RBQ-1, SNAMA)
  • Promoter mouse U6 promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Esp3I (destroyed during cloning)
  • 3′ cloning site Esp3I (destroyed during cloning)
  • 5′ sequencing primer GAGGCTTAATGTGCGATAAAAGA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PB-2G-CNCB_sgRBBP6-68 was a gift from Kazutoshi Takahashi (Addgene plasmid # 237555 ; http://n2t.net/addgene:237555 ; RRID:Addgene_237555)

  • For your References section:

    EIF3D safeguards the homeostasis of key signaling pathways in human primed pluripotency. Okubo C, Nakamura M, Sato M, Shichino Y, Mito M, Takashima Y, Iwasaki S, Takahashi K. Sci Adv. 2025 Apr 11;11(15):eadq5484. doi: 10.1126/sciadv.adq5484. Epub 2025 Apr 9. 10.1126/sciadv.adq5484 PubMed 40203091
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