PNLM-pcABE
(Plasmid
#237618)
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PurposePNLM-pcABE generated by pre-trained Protein-Nucleic Acid constrained Language Model exhibits high efficiency, a condensed editing window, and a shortened size.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 237618 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backboneCustom
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Vector typeMammalian Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameecTadA(8e)-nSpCas9
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SpeciesH. sapiens (human)
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Insert Size (bp)457
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Mutationdeleted amino acids 2-8 and 147-152
- Promoter pCMV
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer pCMV-F
- 3′ sequencing primer BGH-R
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PNLM-pcABE was a gift from Xiaohui Zhang (Addgene plasmid # 237618 ; http://n2t.net/addgene:237618 ; RRID:Addgene_237618) -
For your References section:
Protein-nucleic acid language model-assisted design of precise and compact adenine base editor. Ren J, Yao J, Cao Q, Li Y, Li Y, Zhang Z, Ge X, Wang S, Zhang Y, Wang X, Zhang X. Nat Commun. 2025 Dec 13;16(1):11207. doi: 10.1038/s41467-025-65311-z. 10.1038/s41467-025-65311-z PubMed 41390734
Map uploaded by the depositor.
