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pORTMAGE-Sa2
(Plasmid #237793)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 237793 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pRAB11
  • Vector type
    Bacterial Expression ; Recombineering in Staphylococcus aureus
  • Selectable markers
    Ampicillin for E. coli maintenance.

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Staphylococcus aureus RN4220
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    C41pRecT
  • Species
    Clostridium sp. FS41.
  • Insert Size (bp)
    657
  • GenBank ID
    WP_152649059

Gene/Insert 2

  • Gene/Insert name
    C41pExo
  • Species
    Clostridium sp. FS41.
  • Insert Size (bp)
    822

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pORTMAGE-Sa2 was a gift from Timothy Wannier (Addgene plasmid # 237793)

  • For your References section:

    A diverse single-stranded DNA-annealing protein library enables efficient genome editing across bacterial phyla. Filsinger GT, Mychack A, Lyerly E, Henriksen C, Bartlett TM, Kuchwara H, Eitzinger S, Bernhardt TG, Walker S, Church GM, Wannier TM. Proc Natl Acad Sci U S A. 2025 Apr 29;122(17):e2414342122. doi: 10.1073/pnas.2414342122. Epub 2025 Apr 21. 10.1073/pnas.2414342122 PubMed 40258142
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