pORTMAGE-Cg1
(Plasmid
#237794)
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PurposeSingle-stranded and double-stranded recombineering in Corynebacterium glutamicum
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 237794 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTG5
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Vector typeRecombineering in Corynebacterium glutamicum
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)Corynebacterium glutamicum
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert nameTspRecT
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SpeciesTreponema socranskii subsp. socranskii
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Insert Size (bp)819
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GenBank IDWP_021329327
Gene/Insert 2
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Gene/Insert nameTspSSB
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SpeciesTreponema socranskii subsp. socranskii
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Insert Size (bp)537
Resource Information
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Supplemental Documents
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pORTMAGE-Cg1 was a gift from Timothy Wannier (Addgene plasmid # 237794) -
For your References section:
A diverse single-stranded DNA-annealing protein library enables efficient genome editing across bacterial phyla. Filsinger GT, Mychack A, Lyerly E, Henriksen C, Bartlett TM, Kuchwara H, Eitzinger S, Bernhardt TG, Walker S, Church GM, Wannier TM. Proc Natl Acad Sci U S A. 2025 Apr 29;122(17):e2414342122. doi: 10.1073/pnas.2414342122. Epub 2025 Apr 21. 10.1073/pnas.2414342122 PubMed 40258142