pAG CMV C-HA Puro
(Plasmid
#237880)
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Purpose(Empty Backbone) Empty mammalian expression plasmid for cloning C-terminally HA-tagged proteins
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Depositing Labs
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 237880 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepAG
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Backbone manufacturerAddgene
- Backbone size (bp) 4199
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Vector typeMammalian Expression
- Promoter CMV
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Selectable markersPuromycin
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Tag
/ Fusion Protein
- HA (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer KS primer (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAG CMV C-HA Puro was a gift from Meghan Rego & Addgene Research Program (Addgene plasmid # 237880 ; http://n2t.net/addgene:237880 ; RRID:Addgene_237880)
Map uploaded by the depositor.
