pAAV-TRE-fDIO-mem-seTurboID-IRES-tTA
(Plasmid #237889)

• Purpose: Amplifier AAV vector of Dual-AAV sparse labeling system encoding membrane-tethered seTurboID
• Depositing Lab: Rui Lin
• Publication: Zhong et al Neuron. 2025 Sep 18:S0896-6273(25)00657-9. doi: 10.1016/j.neuron.2025.08.022.

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• Serotype: Select serotype for details See details about
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Backbone

• Vector backbone: pAAV
• Total vector size (bp): 7176
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mem-seTurboID
• Species: Synthetic
• Insert Size (bp): 1242
• Promoter: TRE
• Tags / Fusion Proteins:
  • FLAG tag (N terminal on insert)
  • GAP43 palmitoylation signal (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: AAGACAGGGCCAGGTTTCCG

Resource Information

• Supplemental Documents:
  • pAAV-TRE-fDIO-mem-seTurboID-IRES-tTA.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry