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pEGFP-AAK1-Ser624Ala
(Plasmid #237899)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 237899 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pEGFPC3
  • Backbone manufacturer
    Clonetech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 7584
  • Modifications to backbone
    Serine residue at 625 is mutated to Alanine
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AP2-associated protein kinase 1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2880
  • Mutation
    Serine 624 changed to alanine
  • GenBank ID
    NM_014911.5
  • Entrez Gene
    AAK1
  • Promoter CMV
  • Tag / Fusion Protein
    • GFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xhoi (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer CATGGTCCTGCTGGAGTTCGTG
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-AAK1-Ser624Ala was a gift from Eleanor Coffey (Addgene plasmid # 237899 ; http://n2t.net/addgene:237899 ; RRID:Addgene_237899)
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