Dendra2-NS3
(Plasmid
#238004)
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PurposeExpresses NS3 in mammalian/mouse cell line
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 238004 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneDendra2 Tubulin
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNS3
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SpeciesDengue Virus Serotype 2
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Insert Size (bp)1800
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Tag
/ Fusion Protein
- Dendra2 (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Sac1 (not destroyed)
- 3′ cloning site BamH1 (not destroyed)
- 5′ sequencing primer CMV-Forward
- 3′ sequencing primer SV40-pArev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made bypcDNA_DENV2-NS2B3_V5. https://www.addgene.org/115906/
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
A portion of this plasmid was derived from a plasmid made by: pcDNA_DENV2-NS2B3_V5. https://www.addgene.org/115906/
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Dendra2-NS3 was a gift from Partha Mondal (Addgene plasmid # 238004 ; http://n2t.net/addgene:238004 ; RRID:Addgene_238004)