pAAV-EF1α-DIO-cOpn5-T2A-mcherry
(Plasmid
#238009)
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PurposeThe transfer plasmid for packaging AAV expressing Cre-dependent chicken OPN5 was constructed by inserting the OPN5 coding sequence into the multiple cloning site under the control of EF1α promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 238009 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepAAV
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Vector typeAAV, Cre/Lox
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameOpn5
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SpeciesG. gallus (chicken)
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Entrez GeneOPN5 (a.k.a. GPR136, GRP136, Opn5m, PGR12, TMEM13, cOpn5m, neuropsin, opsin-5)
- Promoter EF1α
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-EF1α-DIO-cOpn5-T2A-mcherry was a gift from Minmin Luo (Addgene plasmid # 238009 ; http://n2t.net/addgene:238009 ; RRID:Addgene_238009) -
For your References section:
A neuropsin-based optogenetic tool for precise control of Gq signaling. Dai R, Yu T, Weng D, Li H, Cui Y, Wu Z, Guo Q, Zou H, Wu W, Gao X, Qi Z, Ren Y, Wang S, Li Y, Luo M. Sci China Life Sci. 2022 Jul;65(7):1271-1284. doi: 10.1007/s11427-022-2122-0. Epub 2022 May 12. 10.1007/s11427-022-2122-0 PubMed 35579776