Skip to main content
Addgene
Open Menu

pIVT-A3A-DEN2
(Plasmid #238019)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 238019 Standard format: Plasmid sent in bacteria as agar stab 1 $89
Add to Cart

Backbone

  • Vector backbone
    pnCas9-PBE
  • Backbone manufacturer
    Caixia Gao, Addgene Plasmid #98164
  • Vector type
    Plant Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL10 Gold
  • Growth instructions
    While the plasmid can be maintained in DH5alpha, please note that the polyA sequence on the plasmid may experience partial adenine (A) loss during E. coli amplification. Therefore, it's essential to pick single colonies for sequencing verification after expansion. The depositing lab specifically selected the XL10 strain because it demonstrates relatively less polyA sequence loss compared to other strains.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    A3A-nSpCas9(D10A)-UGI
  • Species
    Other
  • Mutation
    D10A for SpCas9
  • Promoter T7

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIVT-A3A-DEN2 was a gift from Caixia Gao (Addgene plasmid # 238019 ; http://n2t.net/addgene:238019 ; RRID:Addgene_238019)

  • For your References section:

    An efficient mRNA delivery system for genome editing in plants. Qiu F, Xue C, Liu J, Li B, Gao Q, Liang R, Chen K, Gao C. Plant Biotechnol J. 2025 Apr;23(4):1348-1358. doi: 10.1111/pbi.14591. Epub 2025 Feb 10. 10.1111/pbi.14591 PubMed 39928528
Related items: