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Addgene

ADEPT-pCas9
(Plasmid #238032)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 238032 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    ADEPT-pCas9
  • Backbone size w/o insert (bp) 3515
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    spCas9
  • Species
    S. pyogenes
  • Insert Size (bp)
    4104
  • Promoter tet
  • Tag / Fusion Protein
    • ssrA (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    pwtCas9-bacteria (Addgene plasmid #44250)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The plasmid has a low-copy origin of replication (sc101) but has not exhibited difficulty in growth or miniprep.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ADEPT-pCas9 was a gift from Lingchong You (Addgene plasmid # 238032 ; http://n2t.net/addgene:238032 ; RRID:Addgene_238032)

  • For your References section:

    Population-level amplification of gene regulation by programmable gene transfer. Son HI, Hamrick GS, Shende AR, Kim K, Yang K, Huang TJ, You L. Nat Chem Biol. 2025 Jan 8. doi: 10.1038/s41589-024-01817-9. 10.1038/s41589-024-01817-9 PubMed 39779901
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