ADEPT-pTarget
(Plasmid
#238033)
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PurposeEncodes sfGFP-SsrA under constitutive promoter (J23119). Expresses a single guide RNA, which targets a noncoding region of the plasmid as part of the ADEPT system. Carries oriT and kan resistance.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 238033 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneADEPT-pTarget
- Backbone size w/o insert (bp) 3283
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesfGFP
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SpeciesSynthetic
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Insert Size (bp)714
- Promoter J23119 (constitutive)
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Tag
/ Fusion Protein
- ssrA (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
gRNA sequence: guuuuagagcuagaaauagcaaguuaaaauaaggcuaguccguuaucaacuugaaaaaguggcaccgagucggugc
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ADEPT-pTarget was a gift from Lingchong You (Addgene plasmid # 238033 ; http://n2t.net/addgene:238033 ; RRID:Addgene_238033) -
For your References section:
Population-level amplification of gene regulation by programmable gene transfer. Son HI, Hamrick GS, Shende AR, Kim K, Yang K, Huang TJ, You L. Nat Chem Biol. 2025 Jan 8. doi: 10.1038/s41589-024-01817-9. 10.1038/s41589-024-01817-9 PubMed 39779901