TTR C10S S85C F87E
(Plasmid
#238074)
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PurposeBacterial expression of human TTR mutants
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 238074 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET29a
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Backbone manufactureremdmillipore
- Backbone size w/o insert (bp) 5371
- Total vector size (bp) 5758
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameTTR
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SpeciesH. sapiens (human)
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Insert Size (bp)387
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MutationC10S S85C F87E
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Entrez GeneTTR (a.k.a. ATTR, CTS, CTS1, HEL111, HsT2651, PALB, TBPA, TTN)
- Promoter T7
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
We made mutations on the wild type construct received from the Kelly lab. Description of wild type TTR plasmid generation in supplementary info of this reference : https://pubs.acs.org/doi/abs/10.1021/cb5004477
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
TTR C10S S85C F87E was a gift from Peter Wright (Addgene plasmid # 238074 ; http://n2t.net/addgene:238074 ; RRID:Addgene_238074) -
For your References section:
Conformational Dynamics of an Amyloidogenic Intermediate of Transthyretin: Implications for Structural Remodeling and Amyloid Formation. Leach BI, Ferguson JA, Morgan G, Sun X, Kroon G, Oyen D, Dyson HJ, Wright PE. J Mol Biol. 2024 Aug 15;436(16):168673. doi: 10.1016/j.jmb.2024.168673. Epub 2024 Jun 21. 10.1016/j.jmb.2024.168673 PubMed 38909653