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TTR F87E
(Plasmid #238075)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 238075 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET29a
  • Backbone manufacturer
    emdmillipore
  • Backbone size w/o insert (bp) 5371
  • Total vector size (bp) 5758
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    TTR
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    387
  • Mutation
    F87E
  • Entrez Gene
    TTR (a.k.a. ATTR, CTS, CTS1, HEL111, HsT2651, PALB, TBPA, TTN)
  • Promoter T7

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

We made mutations on the wild type construct received from the Kelly lab. Description of wild type TTR plasmid generation in supplementary info of this reference : https://pubs.acs.org/doi/abs/10.1021/cb5004477

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TTR F87E was a gift from Peter Wright (Addgene plasmid # 238075 ; http://n2t.net/addgene:238075 ; RRID:Addgene_238075)

  • For your References section:

    Conformational Dynamics of an Amyloidogenic Intermediate of Transthyretin: Implications for Structural Remodeling and Amyloid Formation. Leach BI, Ferguson JA, Morgan G, Sun X, Kroon G, Oyen D, Dyson HJ, Wright PE. J Mol Biol. 2024 Aug 15;436(16):168673. doi: 10.1016/j.jmb.2024.168673. Epub 2024 Jun 21. 10.1016/j.jmb.2024.168673 PubMed 38909653
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