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AAV-CMV-Vcan-V3-HA
(Plasmid #238076)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 238076 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pAAV
  • Backbone size w/o insert (bp) 4082
  • Total vector size (bp) 6083
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Vcan
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2054
  • GenBank ID
    NM_001134474
  • Entrez Gene
    Vcan (a.k.a. 5430420N07Rik, 9430051N09, Cspg2, DPEAAE, NG2, PG-M, PG-M(V0), PG-M(V1), hdf)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer aagcagagctCCTCGAGAAGcggccgcactactccgcggactactactagtTTCACGCGGGCCGCCatgttgataaatatgaaagg
  • 3′ sequencing primer CCGCCCGGGTCGACTCTAGAGGTACCtcaagcgtaatctggaacatcgtatgggtaggatccgcgcctggtttcct
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAV-CMV-Vcan-V3-HA was a gift from Yuxuan Guo (Addgene plasmid # 238076 ; http://n2t.net/addgene:238076 ; RRID:Addgene_238076)

  • For your References section:

    Neural Organoids Protect Engineered Heart Tissues From Glucolipotoxicity by Transferring Versican in a Co-Culture System. Bai B, Li J, Wang Z, Yang Y, He J, Chen G, Zhang Y, Qi Y, Wan Z, Cai L, Wang R, Wang K, Zhao D, Zhang J, Huang W, Xu RX, Sun M, Han X, Liu Y, Zhang D, Zhu W, Liu J, Guo Y. Cell Prolif. 2025 Jun 3:e70070. doi: 10.1111/cpr.70070. 10.1111/cpr.70070 PubMed 40459298
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