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Addgene

Mito-PAmKate
(Plasmid #238078)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 238078 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PS-CFP2-Mito-7
  • Modifications to backbone
    Removed PS-CFP2 and inserted PAmKate.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PAmKate
  • Insert Size (bp)
    715
  • Promoter CMV
  • Tag / Fusion Protein
    • Mito (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV Forward
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Mito-PAmKate was a gift from Partha Mondal (Addgene plasmid # 238078 ; http://n2t.net/addgene:238078 ; RRID:Addgene_238078)

  • For your References section:

    Super-resolution optical microscopy reveals accumulation of photoactivable dengue protein (Dendra2-NS2B) in the endoplasmic reticulum. Aravinth S, Varghese JM, Pant N, Mondal PP. Sci Rep. 2025 Mar 26;15(1):10358. doi: 10.1038/s41598-025-94135-6. 10.1038/s41598-025-94135-6 PubMed 40133359
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