ABE8e-YA
(Plasmid
#238085)
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PurposeA High-Fidelity Adenine Base Editor with YA Context Preference
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 238085 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonePCMV
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Vector typeMammalian Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameTadA-8e (A48E)-nSpCas9
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SpeciesH. sapiens (human)
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Insert Size (bp)4818
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-R
- (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.11.23.624961 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ABE8e-YA was a gift from Xiaohui Zhang (Addgene plasmid # 238085 ; http://n2t.net/addgene:238085 ; RRID:Addgene_238085) -
For your References section:
A motif preferred adenine base editor with minimal bystander and off-targets editing. Shang M, Li Y, Cao Q, Ren J, Zeng Y, Wang J, Gonzalez RVL, Zhang X. Nat Commun. 2025 Oct 15;16(1):9153. doi: 10.1038/s41467-025-64203-6. 10.1038/s41467-025-64203-6 PubMed 41093849