pLV-EF1A-PARP14-1535-1601-Hygro
(Plasmid
#238100)
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PurposeExpression of structurally predicted WWE domain of PARP14 with EGFP on C terminal
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 238100 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backboneLentiviral
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Vector typeMammalian Expression, Lentiviral
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePARP14
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SpeciesH. sapiens (human)
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Insert Size (bp)969
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Mutationaa 1535-1601 only
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Entrez GenePARP14 (a.k.a. ARTD8, BAL2, PARP-14, pART8)
- Promoter EF1alpha
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Tag
/ Fusion Protein
- EGFP (C terminal on insert)
Cloning Information
- Cloning method Other
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byVectorBuilder
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLV-EF1A-PARP14-1535-1601-Hygro was a gift from Robert Sobol (Addgene plasmid # 238100 ; http://n2t.net/addgene:238100 ; RRID:Addgene_238100) -
For your References section:
Overexpression of the WWE domain of RNF146 modulates poly-(ADP)-ribose dynamics at sites of DNA damage. Al-Rahahleh RQ, Roos WP, Saville KM, Andrews JF, Wu Z, Koczor CA, Prakash A, Sobol RW. DNA Repair (Amst). 2025 Jun;150:103845. doi: 10.1016/j.dnarep.2025.103845. Epub 2025 May 21. 10.1016/j.dnarep.2025.103845 PubMed 40403420